Detection and prevention of mycoplasma hominis infection
AssigneesThe University of Scranton, Research Corporation Technologies, Inc.
InventorsVito G. DelVecchio, Gary L. Gallia, Ferne K. McCleskey
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Abstract
The present invention is directed to a rapid and sensitive method for detecting Mycoplasma hominis using M. hominis-specific probes, oligonucleotides or antibodies. In particular a target sequence can be amplified by in vitro nucleic acid amplification techniques, detected by nucleic acid hybridization using the subject probes and oligonucleotides or detected by immunoassay using M. hominis-specific antibodies. M. hominis-specific nucleic acids which do not recognize or hybridize to genomic nucleic acid of other Mycoplasma species are also provided.
Claims76
A method of detecting Mycoplasma hominis comprising:
- a) amplifying a Mycoplasma hominis target nucleic acid by contacting a sample to be tested for the presence of Mycoplasma hominis with at least one oligonucleotide for a time and under conditions sufficient to produce copies of said target nucleic acid, wherein said oligonucleotide consists of at least 14 contiguous nucleotides of Mycoplasma hominis DNA of pMhom120 having ATCC Accession Number 97512, or wherein said oligonucleotide has at least about 70% complementarity to at least 14 contiguous nucleotides of SEQ ID NO:1 or SEQ ID NO:3, such that said complementarity is sufficient to permit said amplification, and wherein said oligonucleotide does not hybridize to genomic DNA of Ureaplasma urealyticum, Mycoplasma genitalium, Mycoplasma hyorhinis, Mycoplasma orale, Mycoplasma pneumoniae or Mycoplasma salivarium;
- and
- b) detecting said nucleic acid copies, thereby detecting Mycoplasma hominis.
A method of detecting Mycoplasma hominis comprising:
- a) contacting a Mycoplasma hominis target nucleic acid in a sample to be tested for the presence of Mycoplasma hominis with at least one oligonucleotide probe for a time and under conditions to permit hybridization between said oligonucleotide probe and said nucleic acid target, wherein said oligonucleotide probe consists of at least 14 contiguous nucleotides of Mycoplasma hominis DNA of pMhom120 having ATCC Accession Number 97512, or wherein said probe has at least about 70% complementarity to at least 14 contiguous nucleotides of SEQ ID NO:1 or SEQ ID NO:3, such that said probe is sufficiently complementary to hybridize to said target nucleic acid, and wherein said oligonucleotide probe does not hybridize to genomic DNA of Ureaplasma urealyticum, Mycoplasma genitalium, Mycoplasma hyorhinis, Mycoplasma orale, Mycoplasma pneumoniae or Mycoplasma salivarium;
- and
- b) detecting said hybridization thereby detecting said Mycoplasma hominis.
A method of detecting Mycoplasma hominis comprising:
- a) amplifying a Mycoplasma hominis target nucleic acid by contacting a sample to be tested for the presence of Mycoplasma hominis with at least one oligonucleotide for a time and under conditions sufficient to produce copies of said target nucleic acid, wherein said oligonucleotide consists of at least 14 contiguous nucleotides of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6 or SEQ ID NO:7, or wherein said oligonucleotide has at least about 70% complementarity to at least 14 contiguous nucleotides of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6 or SEQ ID NO:7, such that said complementarity is sufficient to permit said amplification, and wherein said oligonucleotide does not hybridize to genomic DNA of Ureaplasma urealyticum, Mycoplasma genitalium, Mycoplasma hyorhinis, Mycoplasma orale, Mycoplasma pneumoniae or Mycoplasma salivarium;
- and
- b) detecting said nucleic acid copies, thereby detecting said Mycoplasma hominis.
A method of detecting Mycoplasma hominis comprising:
- a) contacting a Mycoplasma hominis target nucleic acid in a sample to be tested for the presence of Mycoplasma hominis with at least one oligonucleotide probe for a time and under conditions to permit hybridization between said oligonucleotide probe and said nucleic acid target, wherein said oligonucleotide probe consists of at least 14 contiguous nucleotides of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6 or SEQ ID NO:7, or wherein said probe has at least about 70% complementarity to at least 14 contiguous nucleotides of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6 or SEQ ID NO:7, such that said probe is sufficiently complementary to hybridize to said target nucleic acid, and wherein said oligonucleotide does not hybridize to genomic DNA of Ureaplasma urealyticum, Mycoplasma genitalium, Mycoplasma hyorhinis, Mycoplasma orale, Mycoplasma pneumoniae or Mycoplasma salivarium;
- and
- b) detecting said nucleic acid copies, thereby detecting said Mycoplasma hominis.
An isolated nucleic acid consisting of at least 14 contiguous nucleotides of Mycoplasma hominis DNA of pMhom120 having ATCC Accession Number 97512, or having at least about 70% complementarity to at least 14 contiguous nucleotides of SEQ ID NO:1 or SEQ ID NO:3, and wherein said oligonucleotide does not hybridize to genomic DNA of Ureaplasma urealyticum, Mycoplasma genitalium, Mycoplasma hyorhinis, Mycoplasma orale, Mycoplasma pneumoniae or Mycoplasma salivarium.
An isolated nucleic acid consisting of at least 14 contiguous nucleotides of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6 or SEQ ID NO:7, or having at least about 70% complementarity to at least 14 contiguous nucleotides of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6 or SEQ ID NO:7, and wherein said oligonucleotide does not hybridize to genomic DNA of Ureaplasma urealyticum, Mycoplasma genitalium, Mycoplasma hyorhinis, Mycoplasma orale, Mycoplasma pneumoniae or Mycoplasma salivarium.
An isolated Mycoplasma hominis nucleic acid having SEQ ID NO:1.
An isolated Mycoplasma hominis nucleic acid having SEQ ID NO:3.
An isolated Mycoplasma hominis nucleic acid having SEQ ID NO:4.
An isolated Mycoplasma hominis nucleic acid having SEQ ID NO:5.
An isolated Mycoplasma hominis nucleic acid having SEQ ID NO:6.
An isolated Mycoplasma hominis nucleic acid having SEQ ID NO:7.
An isolated nucleic acid having the approximately 2000 bp Mycoplasma hominis DNA insert of recombinant plasmid pMhom120 having ATCC Accession Number 97512.
An isolated nucleic acid having an antisense nucleotide sequence of SEQ ID NO:1 wherein said nucleic acid is fully complementary to SEQ ID NO:
- 1.
An isolated nucleic acid consisting of plasmid pMhom120 having ATCC Accession Number 97512.
Dependencies are inferred from the claim text.