Chromophore probe for detection of nucleic acid
AssigneesPharmacyclics, Inc., Board of Trustees, Univ. of TX Sys.
InventorsDarren Magda, Jonathan L. Sessler, Brent Iverson
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Abstract
The present invention is directed to chromophoric probes for use in detecting nucleic acid sequences. More particularly, the invention is directed to probes that comprise a texaphyrin chromophore molecule covalently coupled or conjugated to a site-directing molecule, the site-directing molecule having affinity for a biological receptor or a nucleic acid sequence. In one embodiment of the invention, the probe comprises a texaphyrin chromophore conjugated to an oligonucleotide having complementary binding affinity for a target nucleic acid sequence.
Claims20
A probe for the detection of a target nucleic acid sequence, which probe comprises a texaphyrin chromophore conjugated to an oligonucleotide having complementary binding affinity for said targeted nucleic acid sequence.
A probe for the detection of a target nucleic acid sequence, which probe comprises an oligonucleotide comprising a texaphyrin chromophore on either the 5'- or the 3'-end and an amine on the other end, said oligonucleotide having complementary binding affinity for said target nucleic acid and comprising a partial inverted repeat nucleic acid sequence.
A probe for the detection of a target nucleic acid sequence, which probe comprises an oligonucleotide conjugated to a first texaphyrin, which exhibits fluorescence, on either the 5'- or the 3'-end and also conjugated to a second texaphyrin, which has lower excited state energetics as compared to the first texaphyrin, on the other end;
- said oligonucleotide having complementary binding affinity for said target nucleic acid and comprising a partial inverted repeat nucleic acid sequence.
Dependencies are inferred from the claim text.