Modified N-4 nucleotides for use in amplified nucleic acid hybridization assays
AssigneeChiron Corporation
InventorsMichael S. Urdea, Thomas Horn
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Abstract
Linear or branched oligonucleotide multimers useful as amplifiers in biochemical assays which comprise (1) at least one first single-stranded oligonucleotide unit that is complementary to a single-stranded oligonucleotide sequence of interest, and (2) a multiplicity of second single-stranded oligonucleotide units that are complementary to a single-stranded labeled oligonucleotide. Amplified sandwich nucleic acid hybridizations and immunoassays using the multimers are exemplified.
Claims5
A nucleic acid having the structure ##STR12## where Z is a nucleophile, R.sup.1 is a protective group that is base-stable and acid sensitive, R.sup.2 is methyl, R.sup.3 is a protective group that can be removed and replaced with hydrogen without affecting R.sup.1, R.sup.5 is a phosphorus derivative that enables addition of nucleotides to the 5' position of an oligonucleotide chain during chemical synthesis, R.sup.6 is methyl, hydrogen, I, Br or F, and X is an integer in the range of 1 to 8, inclusive.
A nucleic acid having the structure ##STR13## where Z is a nucleophile, R.sup.1 is a protective group that is generally base-stable and acid sensitive, R.sup.2 is hydrogen or methyl, R.sup.3 is a protective group that can be removed and replaced with hydrogen without affecting R.sup.1, R.sup.5 is a phosphorus derivative that enables addition of nucleotides to the 5' position of an oligonucleotide chain during chemical synthesis, R.sup.6 is methyl hydrogen I, Br or F, and X is an integer in the range of 1 to 8, inclusive, wherein R.sup.3 is selected from the group consisting of ##STR14##
Dependencies are inferred from the claim text.