Bacillus thuringiensis delta-endotoxin
AssigneeMycogen Corporation
InventorsMark Thompson, George E. Schwab, H. Ernest Schnepf, Brian Stockhoff
Life of this patent
Estimating the term…
Abstract
An improved Bacillus thuringiensis (B.t.) delta-endotoxin is created by the modification of the gene encoding the toxin. The toxicity of a B.t. toxin was improved by replacing the native protoxin segment with an alternate protoxin segment by constructing a chimeric toxin gene.
Claims7
An isolated DNA molecule comprising a nucleotide sequence encoding a chimeric Bacillus thuringiensis toxin of approximately 1150 to 1200 amino acids, wherein said toxin comprises a crylC core N-terminal toxin portion having a sequence of at least about 600 amino acids and no more than about 1100 amino acids, wherein the amino acid sequence from the end of said core N-terminal sequence to the C-terminus of the chimeric toxin is a crylA(b) C-terminal protoxin portion having a crylA(b) sequence.
A recombinant host transformed to express a chimeric Bacillus thuringiensis toxin comprising a crylC core N-terminal toxin portion and a crylA(b) C-terminal protoxin portion.
Dependencies are inferred from the claim text.