Synthesis of human procollagens and collagens in recombinant DNA systems
AssigneeThomas Jefferson University
InventorsDarwin J. Prockop, Leena Ala-Kokko, Andrzej Fertala, Aleksander Sieron, Kari I. Kivirikko, Amy Geddis, Taina Pihlajaniemi
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Abstract
The invention is transfected cells, substantially all of which contain at least one human collagen gene and express fibrillar collagen molecules derived using methods for synthesizing collagen and collagen fibrils in said cell lines, and methods for treatment of disorders in humans using said collagen derived from said stable cell lines.
Claims23
Recombinant eukaryotic host cells comprising at least one transfected human procollagen or collagen gene, wherein said transfected human collagen or procollagen gene is selected from the group consisting of a gene encoding at least one polypeptide chain of human type I, human type II, or human type III procollagen or collagen, and at least one transfected gene encoding prolyl 4-hydroxylase or a subunit of prolyl 4-hydroxylase.
A method for synthesizing procollagen or collagen in recombinant eukaryotic host cells comprising:
- transfecting at least one human procollagen or collagen gene encoding at least one polypeptide chain selected from the group consisting of human type I collagen or procollagen, human type II collagen or procollagen, and human type III collagen or procollagen into cells;
- transfecting a gene encoding prolyl 4-hydroxylase or a subunit of prolyl 4-hydroxylase into said cells;
- culturing said cells under conditions such that said transfected procollagen or collagen genes are expressed;
- and
- selecting transgected cells that comprise at least one molecule derived from said procollagen or collagen or genes.
Dependencies are inferred from the claim text.