# US 5,591,830 A · Detection of complexes which include basement membrane components as diagnostic of cancer and other diseases

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- Number: US 5,591,830 A
- Document ID: US5591830A
- Status: Granted
- Kind: A
- Application number: 08/456,855
- Filed: 1995-06-01
- Granted: 1997-01-07
- Earliest claimed: 1988-12-12
- Assignees: Bainbridge Sciences, Inc.
- Inventors: Morgan Van Aken; Stefan L. Paskell
- Web page: https://patentgrep.com/patent/US5591830A

## CPC

- C07K 16/3038: {Kidney, bladder}
- G01N 33/54313: {the carrier being characterised by its particulate form}
- G01N 33/5751: of the skin, e.g. melanoma
- G01N 33/57515: of the breast
- G01N 33/5752: of the lungs
- G01N 33/57525: of the liver or pancreas
- G01N 33/57585: {involving compounds identifiable in body fluids}
- G01N 33/576: for hepatitis
- G01N 33/6887: {from muscle, cartilage or connective tissue}
- G01N 2800/34: Genitourinary disorders
- Y10S 436/811: Test for named disease, body condition or organ function
- Y10S 436/813: Cancer
- Y10S 436/82: Hepatitis associated antigens and antibodies
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

Isolated complexes (which include basement membrane components), antibodies to such complexes or polypeptide constituents thereof, and methods for detecting such complexes or constituents are disclosed. Detection of such complexes in a biological sample by immunological and non-immunological methods allows the diagnosis of a variety of diseases, including cancers, collagen degenerative diseases, and hepatitis. Suitable biological samples include urine, cervical secretions, bronchial aspirates, sputum, saliva, feces, serum, synovial fluid, and cerebrospinal fluid.

## Claims

### Claim 1

An antibody that specifically binds to a complex including basement membrane components, said complex being reactive with antibody 1B4 (ATCC No. 11389), and consisting of polypeptides with approximate molecular weights of 165,000; 140,000; 125,000; 98,000; 82,000; 74,000; 55,000; 43,000; 35,000; 26,000; and 16,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

### Claim 2 (depends on 1)

The antibody of claim 1 wherein the antibody is a monoclonal antibody.

### Claim 3

The cell line 1B4, designated by ATCC No. 11389.

### Claim 4 (depends on 3)

A monoclonal antibody produced by the cell line of claim 3.

### Claim 5

An antibody that specifically binds to a complex including basement membrane components, said complex being reactive with antibody 1B4 (ATCC No. 11389), and consisting of polypeptides with approximate molecular weights of 245,000; 190,000; 165,000; 140,000; 125,000; 98,000; 82,000; 74,000; 55,000; 43,000; 35,000; 26,000; and 16,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

### Claim 6 (depends on 5)

The antibody of claim 5 wherein the antibody is a monoclonal antibody.

### Claim 7

An antibody that specifically binds to a polypeptide having an approximate molecular weight of 165,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and to a complex including basement membrane components, said complex being reactive with antibody 1B4 (ATCC No. 11389), and consisting of polypeptides with approximate molecular weights of 165,000; 140,000; 125,000; 98,000; 82,000; 74,000.; 55,000; 43,000; 35,000; 26,000; and 16,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

### Claim 8

An antibody that specifically binds to a polypeptide having an approximate molecular weight of 43,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and to a complex including basement membrane components, said complex being reactive with antibody 1B4 (ATCC No. 11389), and consisting of polypeptides with approximate molecular weights of 165,000; 140,000; 125,000; 98,000; 82,000; 74,000; 55,000; 43,000; 35,000; 26,000; and 16,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

### Claim 9

An antibody that specifically binds to a polypeptide having an approximate molecular weight of 55,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and to a complex including basement membrane components, said complex being reactive with antibody 1B4 (ATCC No. 11389), and consisting of polypeptides with approximate molecular weights of 165,000; 140,000; 125,000; 98,000; 82,000; 74,000; 55,000; 43,000; 35,000; 26,000; and 16,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

### Claim 10

An antibody that specifically binds to a polypeptide having an approximate molecular weight of 82,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and to a complex including basement membrane components, said complex being reactive with antibody 1B4 (ATCC No. 11389), and consisting of polypeptides with approximate molecular weigts of 165,000; 140,000; 125,000; 98,000; 82,000; 74,000; 55,000; 43,000; 35,000; 26,000; and 16,000 as determined by sodium dodecyl sulfate-polyacrylamide gggqel electrophoresis.

### Claim 11

An antibody that specifically binds to a polypeptide having an approximate molecular weight of 98,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and to a complex including basement membrane components, said complex being reactive with antibody 1B4 (ATCC No. 11389), and consisting of polypeptides with approximate molecular weights of 165,000; 140,000; 125,000; 98,000; 82,000; 74,000; 55,000; 43,000; 35,000; 26,000; and 16,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.