Aureobasidium pullulans xylanase, gene and signal sequence
AssigneeUniversity of Georgia Research Foundation, Inc.
InventorsLi Xin-Liang, Lars G. Ljungdahl
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Abstract
A xylanase from Aureobasidium pullulans having a high specific activity is provided as well as a signal protein for controlling excretion into cell culture medium of proteins to which it is attached. DNA encoding these proteins is also provided.
Claims16
An isolated recombinant DNA molecule encoding a xylanase APX-II mature protein having the amino acid sequence set forth in SEQ ID NO:16 from amino acid 1 to 187.
A method for producing xylanase APX-II in a host cell other than Aureobasidium pullulans, said method comprising the steps of:
- (a) infecting or transforming a host cell capable of expressing xylanase coding regions with a vector comprising a promoter active in said host cell operably linked to the coding region for said xylanase APX-II, having an amino acid sequence as set forth in SEO ID NO:16 from amino acid 1 to 187.
An isolated recombinant DNA molecule comprising a nucleotide sequence encoding a signal peptide having an amino acid sequence as set forth in SEQ ID NO:16 from amino acid -34 to -1.
An isolated recombinant DNA molecule wherein a nucleotide sequence encoding a signal peptide having the amino acid sequence as set forth in SEQ ID NO:16 from -34 to -1 is 5' to a heterologous coding sequence.
A method for producing a heterologous protein in a host cell, said method comprising the steps of:
- (a) infecting or transforming said host cell with a vector comprising a promoter active in said host cell operable linked to a signal sequence having an amino acid sequence as set forth in SEQ ID NO:16 from -34 to -1 and a coding sequence which does not encode an amino acid sequence as set forth in SEQ ID NO:16 from amino acid 1 to 187;
- (b) culturing the infected or transformed cell under conditions suitable for gene expression.
Dependencies are inferred from the claim text.