Trehalose-releasing enzyme, and its preparation and uses
AssigneeKabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo
InventorsKazuhiko Maruta, Michio Kubota, Toshiyuki Sugimoto, Toshio Miyake
Life of this patent
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Abstract
Disclosed is a trehalose-releasing enzyme which specifically hydrolyzes the linkage between a trehalose moiety and the remaining glycosyl moiety in a non-reducing saccharide having a trehalose structure as an end unit and having a degree of glucose polymerization of 3 or higher. The molecular weight of the enzyme is about 57,000 to 68,000 daltons on SDS-PAGE, and the isoelectric point is about 3.3 to 4.6 on isoelectrophoresis. The enzyme is useful in an industrial-scale preparation of trehalose, and the trehalose prepared therewith can be readily incorporated into food products, as well as cosmetic- and pharmaceutical-compositions.
Claims25
A process for preparing trehalose, comprising:
- (a) allowing an enzyme to act on a solution containing a non-reducing saccharide having a trehalose structure as an end unit and having a degree of glucose polymerization of 3 or higher to form trehalose, said enzyme being capable of specifically hyudrolyzing the linkage between a trehalose moiety and the remaining glycosly moiety in said non-reducing saccharide;
- (b) purifying the resultant trehalose;
- and
- (c) recovering the purified trehalose.
A process for preparing a saccharide composition containing trehalose, comprising:
- (a) allowing an enzyme to act on a solution containing a non-reducing saccharide having a trehalose structure as an end unit and having a degree of glucose polymerization of 3 or higher to form trehalose, said enzyme being capable of specifically hydrolyzing the linkage between a trehalose moiety and the remaining glycosyl moiety in said non-reducing saccharide;
- and
- (b) recovering the resultant saccharide composition containing trehalose and other saccharide(s).
Dependencies are inferred from the claim text.