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US 5,589,583 AGranted
Plant promoter
AssigneeMonsanto Company
InventorsHarry J. Klee, James S. Elmer
Life of this patent
Estimating the term…
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AssigneeMonsanto Company
InventorsHarry J. Klee, James S. Elmer
Estimating the term…
A plant promoter that is a nucleic acid region located upstream of the 5' end of a plant DNA structural coding sequence that is transcribed at high levels in meristematic tissue and/or rapidly dividing cells. This promoter region is capable of conferring high levels of transcription in meristematic tissue and/or rapidly dividing cells when used as a promoter for a heterologous coding sequence in a chimetic gene. The promoter and any chimeric gene in which it may be used can be used to obtain transformed plants or plant cells. A DNA coding sequence that codes for a gene that is highly transcribed in meristematic tissue of Arabidopsis thaliana is also disclosed. This coding sequence can be used to obtain a cDNA probe useful in obtaining analogous promoters from a homologous coding sequence in other plant species. Chimeric genes including the isolated promoter region, transformed plants containing the isolated promoter region, transformed plant cells and seeds are also disclosed.
An isolated DNA molecule consisting essentially of a plant promoter region from Arabidopsis thaliana located upstream to a structural DNA sequence which hybridizes to a cDNA probe derived from the structural DNA sequence shown in FIG. 1, said plant promoter region, when operably linked to a heterologous gene, causes transcription in meristematic tissue or regions of rapidly dividing plant cells.
A DNA molecule which comprises:
A transformed plant cell which contains a DNA molecule which comprises:
A differentiated plant consisting of plant cells which contain a DNA molecule which comprises:
Dependencies are inferred from the claim text.