Methods and compositions for modulating G protein action
AssigneeBoard of Regents, The University of Texas
InventorsTsutomu Higashijima, Elliott M. Ross
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Abstract
The present disclosure relates to methods and compositions for modulating the action of G proteins. G proteins are important to cellular regulation in all human cells and their function is involved in a wide variety of human disease processes. In particular aspects, the present disclosure concerns the development of mastoparan analogs as well as other amphipathic and cationic peptides, termed receptor-based analogues, that can promote activation of one or more G proteins, and are therefore proposed to be useful in the treatment of a variety of disorders including asthma, ulcers, cardiovascular diseases and even Parkinson's disease.
Claims85
A peptide other than MP, MP-A or MP-T, the peptide being from 12 to 26 amino acids in length and including within its structure a G protein modulator region comprising:
- a) a mastoparan analog region including the following formula:
- AA.sub.1 -AA.sub.2 -AA.sub.3 -AA.sub.4 -AA.sub.5 -AA.sub.6 -AA.sub.7 -AA.sub.8 -AA.sub.9 -AA.sub.10 -AA.sub.11 -AA.sub.12 (Seq id no:
- 70)
- wherein:
- AA.sub.1 =L, W, A, F or Y;
- AA.sub.2 =K, A, R or Q;
- AA.sub.3 =A or K:
- AA.sub.4 -L, W, I, A, K or F;
- AA.sub.5 =A, L, K, C or R;
- AA.sub.6 =A, D or K;
- AA.sub.7 =L, C,, W, A, F or K;
- AA.sub.8 =A, V, K or R;
- AA.sub.9 =K, R, Q or A;
- AA.sub.10 =K, A, R or N;
- AA.sub.11 =L, I,, W, V, A or K;
- and
- AA.sub.12 =L, A or C;
- or
- b) a receptor analog region comprising CVYREAKEQIRKIDRVL (Seq id no:48);
- CVYREAKEQIRKIL (Seq id no:49);
- IVYREAKEQIRKIDRVL (Seq id no:50);
- IVYREAKEQIRKIL (Seq id no:51);
- CIYRETENRARELAALQGSET (Seq id no:52);
- CIYRETENRARELAALQGSETIL (Seq id no:53);
- CVYIVAKRTTKNLEAGVMKEIL (Seq id no:54);
- CVYIVAKRTTKNLEIL (Seq id no:55);
- CVFQVAKRQLQKIDKVL (Seq id no:56);
- CPLSYRAKRTPRRAALM (Seq id no:57);
- CPFRYQSLMTRARAKVI (Seq id no:58);
- REHKALKTLGIIC (Seq id no:59);
- CRSPDFRKAFKRLLC (Seq id no:60);
- CVYREAKEQIRKIDR (Seq id no:61);
- CISRASKSRIKKDKKEPVAIL (Seq id no:62);
- CISRASKSRIKKDKKIL (Seq id no:63);
- CVYVVAKRESRGLKSGLKTDIL (Seq id no:64);
- or CVYVVAKRESRGLKIL (Seq id no:65).
Dependencies are inferred from the claim text.