Cloning of the biosynthetic pathway for chlortetracycline and tetracycline formation and cosmids useful therein
AssigneeAmerican Cyanamid Company
InventorsMichael J. Ryan, Jason A. Lotvin, Nancy Strathy, Susan E. Fantini
Life of this patent
Estimating the term…
Abstract
The present invention describes a purified and isolated nucleic acid molecule which encodes for the biosynthetic pathway of tetracycline, chlortetracycline or an analogue thereof. The invention relates to the isolation and cloning of the nucleic acid molecule in an isolated fragment from Streptomyces aureofaciens and the expression of the biosynthetic gene in a heterologous host such as Streptomyces lividans.
Claims13
A purified and isolated nucleic acid molecule encoding the proteins of the biosynthetic pathway for tetracycline, chlortetracycline, 6-demethylchlortetracycline, demethyltetracycline, 7-chloro-5a, 11a-dehydrotetracycline or 2-decarboxamido-2-acetyltetracycline, wherein said nucleic acid molecule is isolated from an antibiotic-producing wild-type or mutant Streptomyces.
Dependencies are inferred from the claim text.