Oligonucleotides having phosphorothioate linkages of high chiral purity
AssigneeIsis Pharmaceuticals, Inc.
InventorsPhillip D. Cook, Glenn Hoke
Life of this patent
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Abstract
Sequence-specific phosphorothioate oligonucleotides comprising nucleoside units which are joined together by either substantially all Sp or substantially all Rp phosphorothioate intersugar linkages are provided. Such sequence-specific phosphorothioate oligonucleotides having substantially chirally pure intersugar linkages are prepared by enzymatic or chemical synthesis.
Claims18
A phosphorothioate oligonucleotide complementary to a sequence of targeted RNA or DNA wherein at least 75% of the nucleoside units of said oligonucleotide are joined together by Sp phosphorothioate 3' to 5' intersugar linkages.
A phosphorothioate oligonucleotide complementary to a sequence of targeted RNA or DNA wherein at least 75% of the nucleoside units of said oligonucleotide are joined together by Rp phosphorothioate 3' to 5' intersugar linkages.
A phosphorothioate oligonucleotide comprising SEQ ID NO:1, wherein at least 75% of the nucleoside units of said oligonucleotide are joined together by Sp phosphorothioate 3' to 5' intersugar linkages.
A phosphorothioate oligonucleotide comprising SEQ ID NO:4, wherein at least 75% of the nucleoside units of said oligonucleotide are joined together by Rp phosphorothioate 3' to 5' intersugar linkages.
A phosphorothioate oligonucleotide comprising SEQ ID NO:2, wherein at least 75% of the nucleoside units of said oligonucleotide are joined together by Sp phosphorothioate 3' to 5' intersugar linkages.
A phosphorothioate oligonucleotide comprising SEQ ID NO:1, wherein at least 75% of the nucleoside units of said oligonucleotide are joined together by Rp phosphorothioate 3' to 5' intersugar linkages.
A phosphorothioate oligonucleotide comprising SEQ ID NO:4, wherein all nucleoside units of said oligonucleotide are joined together by Rp phosphorothioate 3' to 5' intersugar linkages.
A phosphorothioate oligonucleotide comprising SEQ ID NO:2, wherein at least 75% of the nucleoside units of said oligonucleotide are joined together by Rp phosphorothioate 3' to 5' intersugar linkages.
Dependencies are inferred from the claim text.