Vaccine against Pasteurella
AssigneeBritish Technology Group Limited
InventorWilliam Donachie
Life of this patent
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Abstract
A vaccine against Pasteurella comprising a proteinaceous material isolated from Pasteurella grown under iron-restricted conditions, but not from Pasteurella grown under normal conditions in vitro, which reacts in an immunoblotting test against the serum of a convalescent sheep or cow which has recovered from an infection by Pasteurella of the same serotype, together with an adjuvant.
Claims15
A vaccine comprising an effective amount of killed whole cells of Pasteurella haemolytica grown under iron-restriction conditions in vitro, together with an adjuvant.
A vaccine comprising an effective mount of killed whole cells of Pasteurella multocida grown under iron-restriction conditions in vitro, other than conditions of attenuation by repeated passaging, together with an adjuvant.
A vaccine comprising an effective mount of killed whole cells of Pasteurella piscicida grown under iron-restriction conditions in vitro, together with an adjuvant.
A vaccine against Pasteurella which comprises an effective amount of a proteinaceous material selected from the group consisting of:
- (a) isolated protein which is isolatable from Pasteurella hacmolytica grown under iron-restriction conditions in vitro but not from said Pasteurella haemolytica grown under normal conditions in vitro, which reacts in an immunoblotting test against serum of a convalescent animal which has recovered from an infection by said Pasteurella haemolytica of the same serotype;
- and
- (b) killed whole cells of Pasteurella haemolytica grown under iron-restriction conditions in vitro;
- said proteinaceous material being formulated together with an adjuvant.
A vaccine against Pasteurella which comprises an effective mount of a proteinaceous material selected from the group consisting of:
- (a) isolated protein which is isolatable from Pasteurella piscicida grown under iron-restriction conditions in vitro but not from said Pasteurella piscicida grown under normal conditions in vitro, which reacts in an immunoblotting test against serum of a convalescent animal which has recovered from an infection by said Pasteurella piscicida of the same serotype;
- and
- (b) killed whole cells of Pasteurella piscicida grown under iron-restriction conditions in vitro;
- said proteinaceous material being formulated together with an adjuvant.
A method of prevention or control of pasteurellosis in sheep or cattle, which method comprises the step of administering to sheep or cattle a prophylactically effective amount of a proteinaceous material selected from the group consisting of
- (a) isolated protein which is isolatable from Pasteurella haemolytica grown under iron-restriction conditions in vitro but not from said Pasteurella haemolytica grown under normal conditions in vitro, which reacts in an immunoblotting test against serum of a convalescent animal which has recovered from an infection by said Pasteurella haemolytica of the same serotype;
- (b) an extract comprising outer membrane proteins of a Pasteurella haemolytica, said extract containing a protein which is isolatable from said Pasteurella haemolytica grown under iron-restriction conditions in vitro but not from said Pasteurella haemolytica grown under normal conditions in vitro, and which reacts in an immunoblotting test against serum of a convalescent animal which has recovered from an infection by said Pasteurella haomolytica of the same serotype;
- and
- (c) killed whole cells of a Pasteurella haemolytica grown under iron-restriction conditions in vitro.
A method of prevention or control of pasteurellosis in sheep or cattle, which method comprises the step of administering to sheep or cattle a prophylactically effective mount of killed whole cells of Pasteurella haemolytica grown under iron-restriction conditions in vitro.
A method of prevention or control of pasteurellosis in cattle, which method comprises the step of administering to cattle a prophylactically effective amount of killed whole cells of Pasteurella multocida grown under iron-restriction conditions in vitro other than conditions of attenuation by repeated passaging.
A method of prevention or control of pasteurellosis in fish, which method comprises administering to fish a prophylactically effective mount of a proteinaceous material selected from the group consisting of
- (a) an isolated protein isolatable from Pasteurella piscicida grown under iron-restriction conditions in vitro but not from said Pasteurella grown under normal conditions in vitro and which resets in an immunoblotting test against serum of a convalescent fish which has recovered from an infection by said Pasteurella piscicida of the same serotype, and
- (b) killed whole cells of Pasteurella piscicida grown under iron-restriction conditions in vitro.
Dependencies are inferred from the claim text.