Production of virus resistant plants
AssigneeState of Oregon acting by and through the State Board of Higher Education on behalf of Oregon State University
InventorsWilliam G. Dougherty, John A. Lindbo
Life of this patent
Estimating the term…
Abstract
A method of suppressing virus gene expression in plants using untranslatable plus sense RNA is disclosed. The method is useful for the production of plants that are resistant to virus infection.
Claims29
A method of producing a transformed plant cell with a reduced capacity to support the expression of a plus-sense RNA virus gene relative to an untransformed plant cell, the method comprising transforming the plant cell with a nucleic acid molecule wherein the nucleic acid molecule comprises a coding sequence operably linked to a promoter sequence, and wherein the coding sequence encodes an untranslatable plus-sense transcript that shares at least 80% nucleotide sequence similarity with a transcript of the plus-sense RNA virus gene.
A method of producing a transformed plant cell with a reduced capacity to support the expression of a potyvirus gene relative to an untransformed plant cell, the method comprising transforming the plant cell with a nucleic acid molecule wherein the nucleic acid molecule comprises a coding sequence operably linked to a promoter sequence, the coding sequence comprising a premature stop codon within 50 nucleotides of a translation initiation codon and encoding an untranslatable plus-sense transcript that shares at least 85% nucleotide sequence similarity with a transcript of the potyvirus gene.
A method of producing a plant with a reduced susceptibility to a plus-sense RNA virus comprising the steps of:
- providing a nucleic acid sequence that shares at least 80% nucleotide sequence similarity with a plus-sense RNA virus gene sequence over a length of at least 100 nucleotides and wherein the nucleic acid sequence encodes a plus-sense RNA molecule;
- modifying the nucleic acid sequence by introducing at least one stop codon or at least one frameshift mutation such that the plus-sense RNA encoded by the modified nucleic acid molecule is untranslatable;
- transforming at least one plant cell with the modified nucleic acid sequence;
- regenerating at least one differentiated plant from the transformed plant cells;
- and
- selecting a regenerated transformed plant that exhibits reduced susceptibility to the plus-sense RNA virus.
A method of producing a plant with a reduced susceptibility to infection by a target plus-sense RNA virus comprising the steps of:
- providing nucleic acid molecules comprising a coding sequence operably linked to a promoter sequence, wherein the coding sequence encodes an untranslatable plus-sense transcript that shares at least 80% nucleotide sequence similarity with a transcript of a gene of the target plus-sense RNA virus;
- transforming plant cells with the nucleic acid molecules;
- growing plants from the transformed plant cells;
- and
- selecting a transformed plant that shows a reduced susceptibility to infection by the target plus-sense RNA virus.
A recombinant DNA molecule effective to inhibit the expression of a potyvirus virus gene when the recombinant DNA molecule and the potyvirus gene are present in a plant cell, the recombinant DNA molecule comprising a coding sequence operably linked to a promoter sequence wherein the coding sequence encodes an untranslatable plus-sense transcript that shares at least 80% nucleotide sequence similarity with a transcript of a potyvirus gene.
Dependencies are inferred from the claim text.