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AssigneesAmerican Cyanamid Company, University of Virginia Patent Foundation
InventorsWilliam R. Baumbach, John R. Zysk, Michael O. Thorner, Bruce D. Gaylinn, Kevin R. Lynch, Jeffrey K. Harrison
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Growth hormone releasing hormone (GHRH) receptor binding has been characterized using a unique binding assay utilizing iodinated GHRH probes. Photoaffinity GHRH probes have been constructed which allow for photolabeling and characterization of the receptor. In addition, high affinity biotinylated GHRH analogs have been constructed. Solubilization of GHRH-R/GHRH complexes and extraction of specifically bound GHRH using a mild detergent solution, followed by affinity chromotography, leads to a substantially purified GHRH-R isolate. Electrophoretic treatment of the GHRH-R isolate produces GHRH-R of sufficient purity to conduct sequencing of the receptor. Cloning of a gene encoding for polypeptides (protein or fragments thereof) having GHRH-R activity is accomplished using a bacterial host, and the cloned gene is expressed in a mammalian cell line.
An isolated and purified nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:9 or its complementary sequence.
A recombinant expression vector comprising a nucleic acid sequence encoding the porcine growth hormone releasing hormone receptor, wherein said nucleic acid sequence is set forth in SEQ ID NO:9, said receptor having a molecular weight of about 52 kD.
Dependencies are inferred from the claim text.