Delivery of exogenous DNA sequences in a mammal
AssigneesVical Incorporated, Wisconsin Alumni Research Foundation
InventorsPhilip L. Felgner, Jon A. Wolff, Gary H. Rhodes, Robert W. Malone, Dennis A. Carson
Life of this patent
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Abstract
Polynucleotide sequences, comprising DNA and RNA molecules can be directly administered, for example by injection, to tissues, such as muscle, and expressed as a protein, polypeptide or polypeptide. The polynucleotides can be contained within liposomes or the polynucleotides can free from association with transfection-facilitating proteins, viral particles, liposomal formulations, charged lipids and calcium phosphate precipitating agents.
Claims20
A method for delivering a desired physiologically active protein, polypeptide or peptide to a mammal, comprising injecting into the muscle of the mammal a non-infectious, non-integrating DNA sequence encoding said protein, polypeptide or peptide operably linked to a promoter, wherein said DNA sequence is free from association with transfection-facilitating proteins, viral particles, liposomal formulations, charged lipids and calcium phosphate precipitating agents, whereby said DNA sequence is expressed.
Dependencies are inferred from the claim text.