Preparations and uses thereof for immunosuppression
AssigneeBoard of Regents, The University of Texas System
InventorsPeter E. Lipsky, Xue L. Tao, Jian Cai
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Abstract
A Tripterygium wilfordii Hook F preparation having an improved LD.sub.50 in mice, an improved therapeutic activity:toxic index ratio and a lower amount of triptolide as compared to previous preparations is disclosed. The LD.sub.50 in mice of the T. wilfordii preparation is greater than about 860 mg/kg, the therapeutic activity:toxic index ratio is greater than about 2.6.times.10.sup.-3, and the amount of triptolide is less than about 1.3 .mu.g/mg. The preparation is useful for immunosuppression, in particular, the suppression of primary antibody response and suppression of autoimmune disease and for the treatment of rheumatoid arthritis.
Claims17
A Tripterygium wilfordii Hook F preparation, obtained from the woody portion of the Tripterygium wilfordii Hook F root, having a triptolide concentration of about 0.2 to about 1.3 .mu.g/mg and having an LD.sub.50 in mice of about 860 to about 1300 mg/kg.
A Tripterygium wilfordii Hook F preparation, obtainable by an alcohol extraction of the woody portion of the Tripterygium wilfordii Hook F root, having a triptolide concentration of about 0.2 to about 1.3 .mu.g/mg.
A Tripterygium wilfordii Hook F preparation, obtainable by an ethanol extraction of the woody portion of the Tripterygium wilfordii Hook F root, having a triptolide concentration of about 0.2 to about 1.3 .mu.g/mg and having an ID.sub.50 in vitro T-cell proliferation/LD.sub.50 ratio of between about 2.6.times.10.sup.-3 to about 4.5.times.10.sup.-3.
A Tripterygium wilfordii Hook F preparation having about 0.2 to about 1.3 .mu.g/mg triptolide obtained by a process comprising the steps of:
- obtaining woody portions of roots of a Tripterygium wilfordii Hook F plant;
- and
- extracting the woody portions with a solvent to produce a Tripterygium wilfordii Hook F preparation;
- wherein the preparation has about 0.2 to about 1.3 .mu.g/mg triptolide.
A Tripterygium wilfordii Hook F preparation having a therapeutic activity:toxic index ratio of between about 2.6.times.10.sup.-3 and about 5.times.10.sup.-3 and having a triptolide concentration of about 0.2 to about 1.3 .mu.g/mg, the preparation obtained by a process comprising the steps of:
- obtaining woody portions of roots of a Tripterygium wilfordii Hook F plant;
- extracting the woody portions with ethanol to produce an ethanol extract;
- and
- extracting the ethanol extract with ethyl acetate to form a Tripterygium wilfordii Hook F preparation;
- wherein the preparation has a therapeutic activity:toxic index ratio of between about 2.6.times.10.sup.-3 and about 5.times.10.sup.-3.
A Tripterygium wilfordii Hook F preparation having a therapeutic activity:toxic index ratio of about 4.5.times.10.sup.-3 and having a triptolide concentration of about 0.2 .mu.g/mg, the preparation obtained by a process comprising the steps of:
- obtaining woody portions of roots of a Tripterygium wilfordii Hook F plant;
- drying the woody portions to form a dried woody portion;
- extracting the dried woody portion with alcohol to produce an alcohol extract;
- and
- extracting the alcohol extract with ethyl acetate to form a Tripterygium wilfordii Hook F preparation;
- wherein the preparation has a therapeutic activity:toxic index ratio about 4.5.times.10.sup.-3 and a triptolide concentration of about 0.2 .mu.g/mg.
Dependencies are inferred from the claim text.