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US 5,578,717 AGranted
Nucleotides for introducing selectably cleavable and/or abasic sites into oligonucleotides
AssigneeChiron Corporation
InventorsMichael S. Urdea, Thomas Horn
Life of this patent
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Abstract
Novel reagents useful in a variety of biochemical and chemical contexts, including nucleic hybridization assays and chemical phosphorylation of hydroxyl-containing compounds. The reagents are particularly useful for introducing cleavable sites and/or abasic sites into oligonucleotide or polynucleotide chains.
Claims11
A compound having the structure
- wherein
- R.sup.1 is a base-stable, acid-sensitive blocking group;
- R.sup.2 is a phosphorous derivative selected to enable addition of the compound to the 5' position of an oligonucleotide chain;
- and
- R is selected from the group consisting of 2-nitrobenzyl, 4-penten-1-yl, ##STR20## in which R' is hydrogen, aryl or aralkyl, the R.sub.i may be the same or different and are selected from the group consisting of amino, nitro, halogeno, hydroxyl, lower alkyl and lower alkoxy, the R.sub.j may be the same or different and are selected from the group consisting of amino, nitro, halogeno, hydroxyl, lower alkyl and lower alkoxy, i is zero, 1, 2 or 3, and j is zero, 1, 2, 3 or 4.
2 more elements
Dependencies are inferred from the claim text.