Composition and methods for transfecting eukaryotic cells
AssigneeLife Technologies Corporation
InventorJoel A. Jessee
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Abstract
The present invention provides compositions and methods for transfecting eukaryotic cells which comprises a nucleic acid, a cationic lipid capable of forming a complex with said nucleic acid, and a viral agent which is an active or inactive enveloped virus or a component of an enveloped virus.
Claims36
A composition for transfecting a eukaryotic cell wherein said composition comprises a nucleic acid, a cationic lipid composition selected from the group consisting of a 3:1 (w/w) mixture of the polycationic lipid, 2,3-dioleyloxy-N-[2(sperminecarboxamido)ethyl]-N,N-dimethyl-1-propanaminiu mtrifluoroacetate and dioleoylphosphatidylethanolamine, and a 1:1 (w/w) mixture of N-[1-(2,3-dioleyloxy)propyl]-N,N,N-trimethylammoniumchloride and dioleoylphosphatidylethanolamine and a viral agent which enhances transfection two-fold or more by said cationic lipid composition, which viral agent is an alphavirus or a component of an alphavirus and wherein said nucleic acid is not a nucleic acid of said viral agent.
Dependencies are inferred from the claim text.