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AssigneeGenencor International, Inc.
InventorsRandy M. Berka, Daniel Cullen, Gregory L. Gray, Kirk J. Hayenga, Virgil B. Lawlis
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Novel vectors are disclosed for expressing and secreting heterologous polypeptides from filamentous fungi. Such vectors are used in novel processes to express and secrete such heterologous polypeptides. The vectors used for transforming a filamentous fungus to express and secrete a heterologous polypeptide include a DNA sequence encoding a heterologous polypeptide and a DNA sequence encoding a signal sequence which is functional in a secretory system in a given filamentous fungus and which is operably linked to the sequence encoding the heterologous polypeptide. Such signal sequences may be the signal sequence normally associated with the heterologous polypeptides or may be derived from other sources. The vector may also contain DNA sequences encoding a promoter sequence which is functionally recognized by the filamentous fungus and which is operably linked to the DNA sequence encoding the signal sequence. Preferably functional polyadenylation sequences are operably linked to the 3' terminus of the DNA sequence encoding the heterologous polypeptides. Each of the above described vectors are used in novel processes to transform a filamentous fungus wherein the DNA sequences encoding the signal sequence and heterologous polypeptide are expressed. The thus synthesized polypeptide is thereafter secreted from the filamentous fungus.
A DNA construct for use in transforming an Aspergillus host to enable secretion an of a heterologous polypeptide, said DNA construct comprising promoter DNA from a fungal gene operably linked to coding DNA, said coding DNA comprising DNA coding for a signal peptide and said heterologous polypeptide.
A transformed Aspergillus expression host capable of secreting a heterologous polypeptide, said host being transformed with a vector comprising promoter DNA from a fungal gene operably linked to coding DNA, said coding DNA comprising DNA coding for a signal peptide and said heterologous polypeptide.
A process for producing a polypeptide comprising:
The process of claim 44 wherein said filamentous fungus is Mucor or Humicola.
The process of claim 44 wherein said gene from said Humicola comprises a glucoamylase gene.
A process for producing a polypeptide comprising:
The process of claims or 46 wherein said heterologous polypeptide comprises an enzyme.
Dependencies are inferred from the claim text.