Mixture of microorganisms, its use for the biodegradation of hydrocarbons, as well as process for its application
AssigneeBiorem AG
InventorsMiroslav Nemec, Dana Horakova
Life of this patent
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Abstract
The mixture of natural microorganisms causes a biodegradation of mineral oils and mineral oil products. It contains Pseudomonas putida and Geotrichum candidum in a ratio of the cell-numbers of 5:1 to 1:1. The mixture is induced by a cultivation in the presence of oil-acid. Afterwards, one works with it on hydrocarbons under the supply of oxygen as well as in the presence of stimulation-substances at a pH-value of 4.5 to 7.5 and at a temperature of 5.degree.+C. to 35.degree. C.
Claims16
A mixture of microorganisms for the biodegradation of hydrocarbons, comprising:
- Pseudomonas putida and Geotrichum candidum in a cell-number ratio of 5:1 to 1:1.
A mixture of microorganisms for the biodegradation of hydrocarbons, comprising:
- Pseudomonas putida and Geotrichum candidum in a cell-number ratio of 5:1 to 1:1;
- and,
- a stimulation substance selected from the group consisting of nitrogen, phosphorous, at least one trace element and a combination thereof.
A process for applying a mixture of microorganisms for a biodegradation of hydrocarbons, comprising the step of:
- preparing a liquid preparation containing between 1-10 grams, per liter of water, of a mixture of microorganisms having Pseudomonas putida and Geotrichum candidum in a cell-number ratio of 5:1 to 1:1, said liquid preparation further including a stimulation substance selected from the group consisting of nitrogen, phosphorous, at least one trace element and a combination thereof;
- and,
- spraying 1-10 liters of said liquid preparation per square meter of soil contaminated by hydrocarbons.
Dependencies are inferred from the claim text.