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AssigneeCell Genesys, Inc.
InventorsRaju Kucherlapati, Beverly H. Koller, Oliver Smithies, Robert B. Dubridge, Gary Greenburg, Daniel J. Capon, Steven R. Williams, Mariona L. A. De Rafael
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Homologous recombination is employed to inactivate genes, particularly genes associated with MHC antigens. Particularly, each of the .beta..sub.2- microglobulin gene and the IFN-.gamma.R gene is inactivated for reducing or eliminating the expression of functional MHC antigens. The resulting cells may be used as universal donor cells. In addition, embryonic stem cells may be modified by homologous recombination for use in producing chimeric or transgenic mammalian hosts, which may be used as source of universal donor organs, or as models for drug and transplantation therapies. Methods for homologous recombination in non-transformed mammalian somatic cells are also described.
A method of producing a genetically engineered mouse lacking functional Class I and II major histocompatibility complex (MHC) antigens, said method comprising:
A genetically engineered mouse characterized by lacking functional class I and II major histocompatibility complex antigens as a result of introduction of a DNA construct into embryonic stem cell producing a lesion that results in inactivation of the IFN.gamma.R gene.
A method for determining the effectiveness of a therapeutic agent in preventing transplant rejection in a mouse, the method comprising:
Dependencies are inferred from the claim text.