3'-RNA labelling with terminal transferase
AssigneeBoehringer Mannheim GmbH
InventorsViola Rosemeyer, Rudolf Seibl, Andreas Laubrock
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Abstract
The present invention concerns a process for introducing non-radioactively labelled deoxynucleotides into nucleic acids and RNA molecules which at their 3' end contain at least one deoxynucleotide which carries a non-radioactive marker group.
Claims43
Process for introducing nucleotides which carry a non-radioactive marker group into nucleic acids, wherein
- at least one non-radioactively labelled deoxynucleotide is attached by terminal deoxynucleotidyl transferase (EC 2.7.7.31) to the 3' end of a nucleic acid acceptor molecule with at least one 3'-terminal ribonucleotide.
Process for introducing marker groups into RNA molecules, wherein
- at least one deoxynucleotide which carries a marker group is attached by terminal deoxynucleotidyl transferase (EC 2.7.7.31) to the 3' end of the RNA molecule.
RNA molecule which, in the region of its 3' end, contains at least one deoxynucleotide which carries a non-radioactive marker group wherein the marker is covalently bound to the nucleotide base of the deoxynucleotide.
Terminal deoxynucleotidyl transferase which shows no measurable RNAse activity at a concentration of up to 50 U/ml terminal deoxynucleotidyl transferase incubated for 4 hours at 37.degree. C.
RNA molecule produced by attaching at least one non-radioactively labelled deoxynucleotide by terminal deoxynucleotidyl transferase to the 3' end of an acceptor RNA molecule, wherein the RNA molecule, in the region of its 3' end, contains at least one deoxynucleotide which carries a non-radioactive marker group wherein the non-radioactive marker group is covalently bound to the nucleotide base of the deoxynucleotide.
Dependencies are inferred from the claim text.