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AssigneeConnaught Laboratories Limited
InventorsJoel Haynes, Michel H. Klein, Benjamin Rovinski, Shi X. Cao
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This invention is directed towards nucleic acid molecules capable of producing human immunodeficiency virus (HIV) retrovirus-like particles, which are non-infectious, replication defective, and immunogenic. Recombinant HIV genomes were generated that are devoid of long terminal repeats (LTRs) but contain a heterologous, inducible metallothionein promoter. Additional modifications have been made to the primer binding site, pol, vif, and env coding regions. Upon transfection into a suitable host these DNA molecules are capable of producing HIV retrovirus-like particles that lack genomic RNA. These non-infectious particles will provide suitable antigens for HIV diagnostic assays and immunogenic preparations.
A DNA molecule comprising an HIV genome devoid of long terminal repeats and a heterologous promoter operatively connected to said HIV genome for expression of said HIV genome in mammalian cells to produce non-infectious, non-replicating and immunogenic HIV retrovirus-like particles.
Dependencies are inferred from the claim text.