Method of N-acylating peptide and proteins with diheteroatom substituted analogs of myristic acid
AssigneeWashington University
InventorsRobert O. Heuckeroth, Steven P. Adams, Jeffrey I. Gordon, George W. Gokel
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Abstract
A method for acylating peptides and proteins with the CoA-ester of diheteroatom substituted C.sub.13 to C.sub.14 myristic acid analogs catalyzed by N-myristoyltransferase is presented. In the analogs, two non-adjacent methylene groups, which are normally at positions 3-13, are replaced with oxygen or sulfur atoms.
Claims2
A method of acylating a peptide or protein at an amino terminal glycine residue of said peptide or protein with an analog of myristic acid to form a N-myristoyl analog peptide or protein comprising reacting said peptide or protein with N-myristoyltransferase and an enzyme substrate consisting of the CoA ester of a diheteroatom-substituted fatty acid analog of myristic acid selected from the group consisting of C.sub.13 and C.sub.14 fatty acids, in which two non-adjacent methylene groups of said analogs which are normally in positions from 3 to 13 are replaced by oxygen or sulfur to reduce the hydrophobicity of said myristic acid analog.
Dependencies are inferred from the claim text.