Production of poly-.beta.-hydroxybutyrate in prokaryotic host cells
AssigneeCenter for Innovative Technology
InventorDouglas E. Dennis
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Abstract
The present invention provides methods for the production of poly-.beta.-hydroxybutyrate, comprising the steps of (a) introducing into a prokaryotic host cell capable of metabolizing sucrose a vector construct which directs the expression of a sequence which encodes a poly-.beta.-hydroxybutyrate biosynthetic pathway, (b) culturing the host cell in medium containing sucrose, and (c) isolating poly-.beta.-hydroxybutyrate from the cultured host cell.
Claims17
A method for the production of poly-.beta.-hydroxybutyrate, comprising:
- (a) introducing into a prokanyotic host cell capable of metabolizing sucrose a vector construct which directs the expression of a sequence which encodes a poly-.beta.-hydroxybutyrate biosynthetic pathway, said prokaryotic host cell selected from the group consisting of E. coli and Klebsiella;
- (b) culturing said host cell in medium containing sucrose;
- and
- (c) isolating poly-.beta.-hydroxybutyrate from said cultured host cell.
A method for the production of poly-.beta.-hydroxyalkanoate copolymer, comprising:
- (a) introducing into a prokaryotic host cell capable of metabolizing sucrose and expressing acetate utilization enzymes constitutively, a vector construct which directs the expression of a sequence which encodes a poly-.beta.-hydroxybutyrate biosynthetic pathway, said prokaryotic host cell selected from the group consisting of E. coli and Klebsiella;
- (b) culturing said host cell in medium containing propionate or a derivative thereof, and sucrose;
- and
- (c) isolating said poly-.beta.-hydroxyalkanoate copolymer from said cultured host cell.
E. coli capable of metabolizing sucrose and containing a vector construct which directs the expression of a sequence which encodes a biosynthetic pathway of poly-.beta.-hydroxybutyrate.
Klebsiella capable of metabolizing sucrose and containing a vector construct which directs the expression of a sequence which encodes a biosynthetic pathway of poly-.beta.-hydroxybutyrate.
A method for the production of poly-.beta.-hydroxybutyrate, comprising:
- (a) introducing into a Klebsiella host cell capable of metabolizing sucrose, a vector construct which directs the expression of a sequence which encodes a poly-.beta.-hydroxybutyrate biosynthetic pathway;
- (b) culturing said host cell in medium containing sucrose;
- and
- (c) isolating poly-.beta.-hydroxybutyrate from said cultured host cell.
A method for the production of poly-.beta.-hydroxyalkanoate copolymer, comprising:
- (a) introducing into a Klebsiella host cell capable of metabolizing sucrose and expressing acetate utilization enzymes constitutively, a vector construct which directs the expression of a sequence which encodes a poly-.beta.-hydroxybutyrate biosynthetic pathway;
- (b) culturing said host cell in medium containing propionate or a derivative thereof, and sucrose;
- and
- (c) isolating said poly-.beta.-hydroxyalkanoate copolymer from said cultured host cell.
Dependencies are inferred from the claim text.