US 5,567,610 A · Method of producing human monoclonal antibodies and kit therefor

Abstract

A method for the removal of, for in vitro immunization, undesired cells from human lymphocyte containing cell populations is disclosed. For the method, use is made of lysosomotropic agents which are caused to act in vitro on human lymphocyte containing cell populations. Also disclosed is a method in the in vitro immunization of lymphocytes for use in the production of human monoclonal antibodies. In this method, lysosomotropic agents are caused to act in vitro on human lymphocyte containing cell populations for removing cell populations having a negative effect on the in vitro immunization, whereupon the lymphocytes are in vitro immunized antigen-specifically and immortalized. Finally, a kit for in vitro immunization of lymphocytes for use in the production of human monoclonal antibodies is disclosed. The kit comprises as active constituents a container containing lymphokines and a container containing lysosomotropic agents.

Claims11

Dependent claims

In an improved method for in vitro immunization of lymphocytes for use in production of human monoclonal antibodies, the improvement comprising:

exposing human peripheral blood lymphocytes to a lysomotrophic agent selected from the group consisting of L-leucine-O-methyl ester, L-glutamic acid dimethyl ester and L-leucyl-L-leucine-O-methyl ester to produce treated human lymphocytes;
and
exposing said treated human lymphocytes to an antigen to activate lymphocytes wherein said treated, activated lymphocytes produce antibody which specifically binds said antigen.

Dependencies are inferred from the claim text.

Facts

Application

08/323,593Filed 17 Oct 1994 · continuation

Priority

SE 86037114 Sep 1986

Granted

22 Oct 1996

Term ends

Not estimatedThe source records don’t support an estimate.

Classification6 codes

C12N 5/0087
Purging biological preparations of unwanted cells: purging against subsets of blood cells, e.g. purging alloreactive T cells
A61K 39/0005
Medicinal preparations containing antigens or antibodies: vertebrate antigens
C12N 5/163
Animal cells: one of the fusion partners being a B or a T lymphocyte
C12N 2501/998
Active agents used in cell culture processes, e.g. differentation: proteins not provided for elsewhere

Method of producing human monoclonal antibodies and kit therefor

Life of this patent

Method of producing human monoclonal antibodies and kit therefor

Life of this patent

Abstract

Claims11