Back to results
US 5,567,599 AGranted
Method for producing transformed chrysanthemum plants
AssigneeFlorigene Europe B.V.
InventorChristine S. Lemieux
Life of this patent
Estimating the term…
Abstract
The invention is directed to a method for introducing an exogenous DNA fragment(s) into a chrysanthemum plant using Agrobacterium as a vector. The invention is further directed to the transformed chrysanthemum plant produced therefrom.
Claims61
A method for producing a chrysanthemum plant comprising an exogenous DNA fragment, which chrysanthemum plant is capable of expressing a gene within the exogenous DNA fragment, comprising the steps of:
- (a) isolating an explant from source material from a chrysanthemum plant, wherein said source material is selected from the group consisting of leaf, stem, peduncle, petiole, meristem, and shoot apex;
- (b) inoculating the explant from source material from the chrysanthemum plant with a culture of Agrobacterium, which Agrobacterium comprises an exogenous DNA fragment comprising a gene under the control of a promoter capable of promoting the transcription of the gene within the exogenous fragment, to obtain an inoculated explant;
- (c) incubating the inoculated explant of step (b) on an incubation medium;
- (d) culturing the incubated explant of step (c) on a regeneration medium to obtain regenerated shoots of the chrysanthemum plant;
- and
- (e) culturing the regenerated shoots of step (d) on a rooting medium to obtain a chrysanthemum plant comprising the exogenous DNA fragment, in which the chrysanthemum plant is capable of expressing the gene within the exogenous DNA fragment.
3 more elements
A method for producing a chrysanthemum plant comprising an exogenous DNA fragment using Agrobacterium as a vector, comprising the steps of:
- (a) incubating chrysanthemum plant source material on a pretreatment medium;
- (b) isolating a leaf explant from source material from the chrysanthemum plant;
- (c) inoculating the explant from source material from the chrysanthemum plant with a culture of Agrobacterium, which Agrobacterium comprises an exogenous DNA fragment comprising a gene under the control of a promoter capable of promoting the transcription of the gene within the exogenous fragment, to obtain an inoculated explant;
- (d) incubating the inoculated explant of step (c) on an incubation medium;
- (e) culturing the incubated mixture of step (d) on a regeneration medium to obtain regenerated shoots of the chrysanthemum plant;
- and
- (f) culturing the regenerated shoots of step (e) on a rooting medium to obtain a chrysanthemum plant comprising the exogenous DNA fragment.
4 more elements
Dependencies are inferred from the claim text.