DNA sequences to target proteins to the mammary gland for efficient secretion
AssigneePharming B.V.
InventorJeffrey M. Rosen
Life of this patent
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Abstract
Described is a method of targeting specific genes to the mammary gland which results in the efficient synthesis and secretion of biologically important molecules. Further, there is described as a composition of matter, a transgenic mammal having the ability to reproduce itself and being suitable for the secretion of biologically active agents into its milk. Additionally there is disclosed as a composition of matter, recombinant DNA gene complexes designed to integrate into a mammalian genome and to synthesize and secrete biological active agents into the milk. Furthermore methods of producing and using altered milk are disclosed.
Claims18
A recombinant DNA complex to provide preferential mammary gland expression and secretion of a selected polypeptide comprising:
- (a) a casein promoter;
- (b) a transcribable DNA segment coding for said selected polypeptide, wherein said promoter does not naturally control the transcription of said DNA segment;
- (c) secretory DNA encoding a signal peptide functional in mammary secretory cells operably linked between said promoter and said DNA segment to facilitate secretion of said selected polypeptide;
- (d) a casein enhancer sequence capable of conferring enhanced preferential transcription of said recombinant DNA complex in mammary secretory cells when said complex is integrated into the genome of a non-human mammal, and
- (e) an intronic sequence from a casein gene or said transcribable DNA segment.
A vector to provide preferential mammary gland expression and secretion of a selected polypeptide comprising:
- (a) a casein promoter;
- (b) a transcribable DNA segment coding for said selected polypeptide, wherein said promoter does not naturally control the transcription of said DNA segment;
- and
- (c) secretory DNA encoding a signal peptide functional in mammary secretory cells operably linked between said promoter and said DNA segment to facilitate secretion of said selected polypeptide,
- (d) a casein enhancer sequence capable of conferring enhanced preferential transcription of said recombinant DNA complex in mammary secretory cells when said complex is integrated into the genome of a non-human mammal, and
- (e) an intron from a casein gene.
A recombinant DNA complex to provide preferential mammary gland expression and secretion of a selected polypeptide comprising:
- (a) a casein promoter;
- (b) a transcribable DNA segment coding for said selected polypeptide, wherein said promoter does not naturally control the transcription of said DNA segment;
- and
- (c) secretory DNA encoding a signal peptide functional in mammary secretory cells operably linked between said promoter and said DNA segment to facilitate secretion of said selected polypeptide,
- (d) a casein enhancer sequence capable of conferring enhanced preferential transcription of said recombinant DNA complex in mammary secretory cells when said complex is integrated into the genome of a non-human mammal,
- (e) an intron from a casein gene;
- and
- (f) a restriction enzyme linker sequence.
A recombinant DNA complex to provide preferential mammary gland expression and secretion of a selected polypeptide comprising:
- (a) a casein promoter;
- (b) a transcribable DNA segment coding for said selected polypeptide, wherein said promoter does not naturally control the transcription of said DNA segment;
- and
- (c) secretory DNA encoding a signal peptide functional in mammary secretory cells operably linked between said promoter and said DNA segment to facilitate secretion of said selected polypeptide,
- (d) a casein enhancer sequence capable of conferring enhanced preferential transcription of said recombinant DNA complex in mammary secretory cells when said complex is integrated into the genome of a non-human mammal, and
- (e) an intron from a casein gene;
- wherein the recombinant DNA complex is linear.
Dependencies are inferred from the claim text.