Method of controlling density of ligand coupled onto supports and products produced therefrom
AssigneeMinnesota Mining and Manufacturing Company
InventorsRaymond M. Gleason, Jerald K. Rasmussen
Life of this patent
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Abstract
A method is disclosed for controlling the coupling low molecular weight ligands to activated sites on a support in competition with a quencher of the activated sites. Derivatized supports produced by the method have optimal ligate binding without overly dense binding sites or stearic effects. Azlactone-functional supports, especially porous supports such as porous particles, benefit from control of ligand density. A linear relationship exists between the molar ratio of ligand and quencher and the coupled ligand density. Optionally, hydrophilicity of the derivatized support can be determined by selection of quencher relative to selection of ligand.
Claims17
A method for controlling density of ligand coupled to an azlactone functional support, comprising the step of reacting ligand and quencher with activated sites on a support under conditions sufficient to promote competition of ligand with quencher for the activated sites,
- wherein the ligand is a molecule having a molecular weight of less than about 1,000 atomic mass units, and wherein the concentration of ligand and the concentration of quencher are within two orders of magnitude;
- wherein there is a linear relationship between the molar ratio of ligand concentration to quencher concentration and density of ligand coupled to the support;
- and wherein there is a linear relationship between molar ratio of ligand concentration to quencher concentration and chromatographic performance of the support.
A derivatized support, comprising an azlactone-functional support having activated sites covalently reacted with ligand and quencher, wherein the density of ligand coupled to the support ranges from about 10% to about 99% of the original activated sites, and wherein there is a linear relationship between molar ratio of ligand concentration to quencher concentration and the chromatographic performance of the support.
Dependencies are inferred from the claim text.