Penton base protein and methods of using same
AssigneeGenVec, Inc.
InventorsThomas J. Wickham, Imre Kovesdi, Douglas E. Brough, Duncan L. McVey, Joseph T. Brader
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Abstract
A recombinant adenovirus comprising a chimeric penton base protein, which includes a nonpenton base sequence, and a therapeutic gene, a method of gene therapy involving the use of such adenovirus, and adenoviral transfer vectors for the generation of such recombinant adenovirus are provided.
Claims35
A recombinant adenovirus comprising (a) a chimeric penton base protein, wherein said chimeric penton base protein comprises a nonpenton base amino acid sequence, which is (i) specific for binding to a receptor, (ii) an antibody domain or (iii) an epitope, in addition to or in place of a wild-type penton base amino acid sequence, which is specific for binding to a receptor, and (b) a therapeutic gene that is capable of being expressed in a cell to which the recombinant adenovirus has attached or by which the recombinant adenovirus has been internalized.
A recombinant baculovirus comprising a chimeric adenoviral penton base gene sequence encoding a chimeric penton base protein, which chimeric adenoviral penton base gene sequence comprises (a) a nonpenton base gene sequence encoding an amino acid sequence that is (i) specific for binding to a receptor, (ii) an antibody domain or (iii) an epitope, and (b) a wild-type penton base gene sequence that encodes an amino acid sequence that is specific for binding to a receptor.
A recombinant baculovirus comprising a chimeric adenoviral penton base gene sequence, wherein said chimeric adenoviral penton base gene sequence includes a nonpenton base gene sequence, which encodes an amino acid sequence that is (i) specific for binding to a receptor, (ii) an antibody domain or (iii) an epitope, in place of a wild-type penton base gene sequence that encodes an amino acid sequence that is specific for binding to a receptor.
A chimeric adenoviral penton base protein comprising a nonpenton base amino acid sequence, which is (i) specific for binding to a receptor, (ii) an antibody domain or (iii) epitope, in addition to or in place of a wild-type penton base amino acid sequence, which is specific for binding to a receptor.
A viral transfer vector comprising a chimeric adenoviral penton base gene sequence encoding a chimeric penton base protein, which chimeric adenoviral penton base gene sequence comprises a nonpenton base gene sequence encoding an amino acid sequence that is (i) specific for binding to a receptor, (ii) an antibody domain or (iii) an epitope, in addition to a wild-type penton base gene sequence that encodes an amino acid sequence that is specific for binding to a receptor.
A viral transfer vector comprising a chimeric adenoviral penton base gene sequence encoding a chimeric penton base protein, which chimeric adenoviral penton base gene sequence comprises a nonpenton base gene sequence encoding an amino acid sequence that is (i) specific for binding to a receptor, (ii) an antibody domain or (iii) an epitope, in place of a wild-type penton base gene sequence that encodes an amino acid sequence that is specific for binding to a receptor.
A prokaryotic or eukaryotic expression vector comprising a chimeric adenoviral penton base gene sequence encoding a chimeric penton base protein, which chimeric adenoviral penton base gene sequence comprises a nonpenton base gene sequence encoding an amino acid sequence that is (i) specific for binding to a receptor, (ii) an antibody domain or (iii) an epitope, in place of a wild-type penton base gene sequence that encodes an amino acid sequence that is specific for binding to a receptor.
A prokaryotic or eukaryotic expression vector comprising a chimeric adenoviral penton base gene sequence encoding a chimeric penton base protein, which chimeric adenoviral penton base gene sequence comprises a nonpenton base gene sequence encoding an amino acid sequence that is (i) specific for binding to a receptor, (ii) an antibody domain or (iii) an epitope, in addition to a wild-type penton base gene sequence that encodes an amino acid sequence that is specific for binding to a receptor.
Dependencies are inferred from the claim text.