Sucrase gene derived from coryneform bacteria
AssigneeAjinomoto Co., Inc.
InventorsMakoto Tsuchiya, Kiyoshi Miwa
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Abstract
The present invention provides a DNA fragment derived from Coryneform bacteria and containing a gene coding for a protein having sucrase activity and a recombinant DNA vector containing said DNA fragment and capable of expression in Coryneform bacteria, The recombinant DNA is introduced into Coryneform bacteria to enhance their sucrase activity, By using the bacteria having enhanced sucrase activity a method is provided for efficiently producing L-amino acids and nucleic acids in a short period of time.
Claims7
A DNA fragment having a nucleic acid sequence encoding the polypeptide encoded by the nucleic acid sequence from base 2,338 to base 3,609 of SEQ ID NO:4.
A method for producing an L-amino acid or a nucleic acid, which comprises
- culturing a Coryneform bacteria which overexpresses a nucleic acid sequence encoding the polypeptide encoded by the nucleic acid sequence from base 2,338 to base 3,609 of SEQ ID NO:4 in a liquid medium containing sucrose, and
- isolating said L-amino acid or nucleic acid from said liquid medium.
Dependencies are inferred from the claim text.