Back to results
US 5,552,538 AGranted
Oligonucleotides with cleavable sites
AssigneeChiron Corporation
InventorsMichael S. Urdea, Thomas Horn
Life of this patent
Estimating the term…
Abstract
Novel reagents useful in a variety of biochemical and chemical contexts, including nucleic hybridization assays and chemical phosphorylation of hydroxyl-containing compounds. The reagents are particularly useful for introducing cleavable sites and/or abasic sites into oligonucleotide or polynucleotide chains.
Claims1
A polynucleotide reagent having the structure
- wherein
- DNA.sub.1 is a first segment of DNA;
- DNA.sub.2 is a second segment of DNA;
- R.sup.3 is selected from the group consisting of hydrogen, methyl, I, Br and F;
- R.sup.4 is hydrogen or methyl;
- and
- R.sup.5 is selected from the group consisting of levulinyl, ##STR20## in which R' is hydrogen, aryl or aralkyl;
- the R.sub.i may be the same or different and are selected from the group consisting of amino, nitro, halogeno, hydroxyl, lower alkyl and lower alkoxy;
- the R.sub.j may be the same or different and are selected from the group consisting of amino, nitro, halogeno, hydroxyl, lower alkyl and lower alkoxy;
- the R.sub.k may be the same or different and are selected from the group consisting of amino, nitro, halogeno, hydroxyl, lower alkyl and lower alkoxy;
- i is zero, 1, 2 or 3, j is zero, 1, 2, 3 or 4, and k is 0, 1, 2, 3 or 4;
- and
- Z is selected from the group consisting of ##STR21## wherein x and y are integers which may be the same or different and are integers in the range of 1 to 8.
10 more elements
Dependencies are inferred from the claim text.