Phosphorothioate inhibitors of metastatic breast cancer
AssigneeBoard of Regents of the University of Nebraska
InventorsEric M. Scholar, Patrick L. Iversen
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Abstract
A method of inhibiting tumor invasion and metastasis of cancer cells using antisense oligonucleotides is disclosed. Phosphorothioate oligos were developed which are complementary to proteolytic enzymes such as urokinase plasminogen activator which are associated with invasion. In vitro and in vivo experiments with these oligos demonstrated highly significant reduction in tumor invasion and metastasis of mammary carcinoma cells.
Claims25
An oligonucleotide selected from the group consisting of:
- SEQ ID NO:3, 4, 8, 13, 14, 15, 16, 17, and 18.
A method for inhibiting basement membrane invasion by a tumor cell in vitro comprising:
- contacting said cell with an oligonucleotide selected from the group consisting of:
- SEQ ID NO:
- 3, 4, 8, 13, 14, 15, 16, 17, and 18, whereby basement membrane invasion is inhibited.
A method for inhibiting tumor cell invasion and metastasis of tumor cells in a mouse, said tumor cells exhibiting increased synthesis or secretion of urokinase plasminogen activator, comprising:
- administering to said mouse an oligonucleotide selected from the group consisting of SEQ ID NO:
- 3 and 4, whereby tumor cell invasion and metastasis are inhibited.
An oligonucleotide wherein said oligonucleotide is SEQ ID NO:3.
An oligonucleotide wherein said oligonucleotide is SEQ ID NO:4.
An oligonucleotide wherein said oligonucleotide is SEQ ID NO:8.
An oligonucleotide wherein said oligonucleotide is SEQ ID NO:13.
An oligonucleotide 33 wherein said oligonucleotide is SEQ ID NO:14.
An oligonucleotide wherein said oligonucleotide is SEQ ID NO:15.
An oligonucleotide wherein said oligonucleotide is SEQ ID NO:16.
An oligonucleotide wherein said oligonucleotide is SEQ ID NO:17.
An oligonucleotide wherein said oligonucleotide is SEQ ID NO:18.
Dependencies are inferred from the claim text.