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AssigneeDekalb Genetics Corporation
InventorsThomas R. Adams, Sheryl A. Chambers, Richard J. Daines, William J. Gordon-Kamm, Albert P. Kausch, Peggy G. Lemaux, Catherine J. Mackey, Mary L. Mangano, James V. O'Brien, Thomas B. Rice, T. Michael Spencer, William G. Start, Nancy G. Willetts
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This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.
A fertile cultivated, transgenic maize plant, the genome of which has been augmented through the genomic introduction of a preselected genetic component, said component comprising an exogenous gene positioned under the control of one or more preselected genetic control elements, the plant prepared by a process comprising:
A fertile, transgenic maize plant, the genetic complement of which has been altered through the addition of a preselected functional genetic element selected from the group consisting of a bar gene;
Dependencies are inferred from the claim text.