Biological adhesive composition and method of promoting adhesion between tissue surfaces
AssigneeOrthogene, Inc.
InventorsKay Juergensen, Daniel Aeschlimann, Ernst B. Hunziker
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Abstract
A formulated biological adhesive composition utilizes tissue transglutaminase in a pharmaceutically acceptable aqueous carrier. The tissue transglutaminase is used in an effective catalytic amount to promote adhesion between tissue surfaces upon treatment thereof by catalyzing the reaction between glutaminyl residues and amine donors of the tissue and/or the enzyme. The carrier contains a divalent metal ion such as calcium to promote said reaction.
Claims36
A formulated biological adhesive composition comprising tissue transglutaminase and a pharmaceutically acceptable carrier, said tissue transglutaminase in an effective amount to promote adhesion upon treatment of tissue in the presence of a divalent metal ion.
A formulated biological adhesive composition comprising tissue transglutaminase and a pharmaceutically acceptable aqueous carrier, said tissue transglutaminase in an amount of about 100 .mu.g/ml to about 50 mg/ml to promote adhesion upon treatment of tissue, said pharmaceutically acceptable aqueous carrier containing calcium ions in a concentration above about 0.5 mM, said composition having a pH of about 7 to about 8.5 and a buffering agent.
A formulated biological adhesive composition comprising tissue transglutaminase and a pharmaceutically acceptable aqueous carrier, said tissue transglutaminase in an amount of about 100 .mu.g/ml to about 50 mg/ml to promote adhesion upon treatment of tissue, said pharmaceutically acceptable aqueous carrier containing calcium ions in a concentration above about 0.5 mM, wherein said tissue comprises a biomatrix having transglutaminase substrate sites and containing cells or an embryo transfer system.
A formulated biological adhesive composition comprising tissue transglutaminase and a pharmaceutically acceptable aqueous carrier, said tissue transglutaminase in an amount of about 100 .mu.g/ml to about 50 mg/ml to promote adhesion upon treatment of tissue, said pharmaceutically acceptable aqueous carrier containing calcium ions in a concentration above about 0.5 mM, for use in the treatment of biomatrix implants further containing a naturally occurring or synthetic protein having transglutaminase substrate sites.
Dependencies are inferred from the claim text.