A device for separating intestines from a cluster of interconnected intestines, gall-bladder and other internal organs, the cluster being fixed at at least one of the other organs, a part thereof or a connection between the organs, the device comprising separating means for breaking one or more tissue connections in the cluster, and having at least one pair of elongated, essentially parallel rollers which are adapted to be rotated in opposite directions to each other, and which rollers are each provided on the outside surface thereof with means for exerting a pulling force on the intestines away from the rest of the cluster, and wherein organ guide means are fitted at the side of the rollers facing the point or points of fixing of the cluster, which organ guide means are adapted to orientate the cluster and partially bound a passage area in the form of a guide slit leading to the rollers, which passage area is large enough to allow the intestines and the gall-bladder through, but is too small to allow adjacent organs to pass.