Cholesterol disposal fusion enzymes
AssigneeThe Regents of the University of California
InventorsWalter L. Miller, Jennifer A. Harikrishna, Stephen M. Black
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Abstract
Fusion enzymes having multiple segments of different biological activity including one segment having P450scc activity and at least one segment having electron-transfer activity for transferring electrons to P450scc are described along with genetic constructs for production of such enzymes and methods for their use. Methods of their use include cholesterol degradation in vitro or in vivo as well as conversion of cholesterol to other useful steroidal products including pregnenolone.
Claims12
A fusion enzyme having an N-terminal end and an C-terminal end, comprising (1) P450scc or a fragment thereof retaining cholesterol-side-chain-cleavage activity and (2) an electron-transfer protein having the ability to transfer electrons to said P450scc.
Dependencies are inferred from the claim text.