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US 5,545,530 AGranted
Process for measuring analyte in sample
AssigneeWako Pure Chemical Industries, Ltd.
InventorsShinji Satomura, Kenji Nakamura, Tokuji Ikenaka, Kaoru Omichi
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Abstract
The amount of an analyte in a sample derived from a living sample is measured by reacting the analyte with an excess of a substance having affinity for the analyte, followed by separation of complex by high pressure liquid chromatography and measurement using a linear calibration curve representing the peak area values associated with known concentrations of analyte.
Claims19
A process for measuring the amount of analyte in a sample device from a living body which comprises:
- mixing the sample with an affinity substance capable of binding the analyte, wherein the affinity substance is present at a concentration 5 or more times as high the concentration of the analyte corresponding to the upper limit of measurement of the analyte, and wherein the affinity substance is either labeled or unlabeled;
- reacting the analyte with the affinity substance to form an analyte-affinity substance complex;
- separating the analyte-affinity substance complex from free affinity substance using high pressure liquid chromatography;
- directly measuring the amount of affinity substance in a fraction eluted during the high pressure liquid chromatography;
- and
- determining the amount of the analyte based upon the amount of affinity substance measured using a linear calibration curve, wherein the linear calibration curve is generated by associating known amounts of analyte with peak area values.
3 more elements
A process for measuring the amount of analyte in a sample derived from a living body which comprises:
- mixing the sample with known amount of labeled analyte and an affinity substance capable of binding the analyte to form a reaction solution, wherein the concentration of labeled analyte is not less than the concentration at which the analyte can bind to the total affinity substance present in the reaction solution;
- incubating the reaction to form labeled analyte-affinity substance complex;
- separating the labeled analyte-affinity substance complex from free labeled analyte using high pressure liquid chromatography;
- directly measuring the amount of labeled analyte-affinity substance complex or the amount of free labeled analyte in a fraction eluted during the high pressure liquid chromatography;
- and
- determining the amount of the analyte based upon the amount of label measured using a linear calibration curve, wherein the linear calibration curve is generated by associating known amounts of analyte with peak area values.
3 more elements
Dependencies are inferred from the claim text.