Nucleic acid encoding synthesis-deficient thermostable DNA polymerase
AssigneeThird Wave Technologies, Inc.
InventorsJames E. Dahlberg, Victor I. Lyamichev, Mary Ann D. Brow
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Abstract
A means for cleaving a nucleic acid cleavage structure in a site-specific manner is disclosed. A cleaving enzyme having 5' nuclease activity without interfering nucleic acid synthetic ability is employed as the basis of a novel method of detection of specific nucleic acid sequences. Cleaving enzymes are produced through the use of novel DNA sequences which encode novel thermostable polymerases.
Claims9
A non-naturally occurring oligonucleotide having a nucleotide sequence encoding a non-naturally occurring thermostable DNA polymerase altered in sequence relative to the naturally occurring sequence of a thermostable DNA polymerase of the genus Thermus such that it exhibits reduced DNA synthetic activity from that of the naturally occurring DNA polymerase, wherein said encoded non-naturally occurring thermostable DNA polymerase exhibits substantially the same 5' nuclease activity as said naturally occurring DNA polymerase.
A recombinant DNA vector comprising DNA having a nucleotide sequence encoding a non-naturally occurring thermostable DNA polymerase altered in sequence relative to the naturally occurring sequence of a thermostable DNA polymerase of the genus Thermus such that it exhibits reduced DNA synthetic activity from that of the naturally occurring DNA polymerase wherein said encoded non-naturally occurring thermostable DNA polymerase exhibits substantially the same 5' nuclease activity as said naturally occurring DNA polymerase.
Dependencies are inferred from the claim text.