Back to results
US 5,539,082 AGranted
Peptide nucleic acids
OwnerPeter E. Nielsen, Ole Buchardt +2inventors
InventorsPeter E. Nielsen, Ole Buchardt, Michael Egholm, Rolf H. Berg
Life of this patent
Estimating the term…
Abstract
A novel class of compounds, known as peptide nucleic acids, bind complementary ssDNA and RNA strands more strongly than a corresponding DNA. The peptide nucleic acids generally comprise ligands such as naturally occurring DNA bases attached to a peptide backbone through a suitable linker.
Claims3
A compound having the formula:
- ##STR8## wherein:
- each L is independently selected from the group consisting of hydrogen, phenyl, heterocyclic moleties, naturally occurring nucleobases, and non-naturally occurring nucleobases;
- each R.sup.3 is independently selected from the group consisting of hydrogen, (C.sub.1 -C.sub.4)alkyl, hydroxy- or alkoxy- or alkylthio-substituted (C.sub.1 -C.sub.4)alkyl, hydroxy, alkoxy, alkylthio and amino;
- each R.sup.7' is independently selected from the group consisting of hydrogen and the side chains of naturally occurring alpha amino acids;
- n is an integer from 1 to 60,
- each k, l, and m is, independently, zero or an integer from 1 to 5;
- each p is zero or 1;
- R.sup.h is OH, NH.sub.2 or --NHLysNH.sub.2 ;
- and
- R.sup.i is H or COCH.sub.3.
7 more elements
A compound having the formula:
- ##STR9## wherein:
- each L is independently selected from the group consisting of hydrogen, phenyl, heterocyclic moieties, naturally occurring nucleobases, and non-naturally occurring nucleobases;
- each R.sup.3 is independently selected from the group consisting of hydrogen, (C.sub.1 -C.sub.4)alkyl, hydroxy- or alkoxy- or alkylthio-substituted (C.sub.1 -C.sub.4)alkyl, hydroxy, alkoxy, alkylthio and amino;
- each R.sup.7' is independently selected from the group consisting of hydrogen and the side chains of naturally occurring alpha amino acids;
- n is an integer from 1 to 60,
- each k, l, and m is, independently, zero or an integer from 1 to 5;
- each p is zero or 1;
- R.sup.h is OH, NH.sub.2 or --NHLysNH.sub.2 ;
- and
- R.sup.i is H or COCH.sub.3.
7 more elements
A compound having the formula:
- ##STR10## wherein:
- L is independently selected from the group consisting of hydrogen, phenyl, heterocyclic moieties, naturally occurring nucleobases, and non-naturally occurring nucleobases;
- R.sup.3 is selected from the group consisting of hydrogen, (C.sub.1 -C.sub.4) alkyl, hydroxy- or alkoxy- or alkylthio-substituted (C.sub.1 -C.sub.4) alkyl, hydroxy, alkylthio and amino;
- each R.sup.7' is independently selected from the group consisting of hydrogen and the side chains of naturally occurring alpha amino acids;
- k, l, and m are, independently, zero or an integer from 1 to 5;
- and
- p is zero or 1.
4 more elements
Dependencies are inferred from the claim text.