Method for preparing fertile transgenic corn plants
AssigneeDekalb Genetics Corporation
InventorsRonald C. Lundquist, David A. Walters
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Abstract
Fertile transgenic Zea mays (corn) plants which stably express heterologous DNA which is heritable are disclosed along with a process for producing said plants. The process comprises the microjectile bombardment of friable embryogenic callus from the plant to be transformed. The process may be applicable to other graminaceous cereal plants which have not proven stably transformable by other techniques.
Claims10
A process for producing a fertile transgenic Zea mays plant comprising the steps of (i) establishing a regenerable embryogenic callus culture from a Zea mays plant to be transformed, (ii) transforming said culture by bombarding it with DNA-coated microprojectiles, (iii) identifying or selecting a transformed cell line, and (iv) regenerating a fertile transgenic Zea mays plant therefrom, wherein said DNA is transmitted through a complete sexual cycle of said transgenic plant to its progeny and imparts herbicide or insect resistance thereto.
A process for producing a fertile transgenic Zea mays plant comprising the steps of (i) establishing a regenerable embryonic callus culture from a Zea mays plant to be transformed, (ii) transforming said culture by bombarding it with DNA-coated microprojectiles, (iii) identifying or selecting a transformed cell line, and (iv) regenerating a fertile transgenic Zea mays plant therefrom, wherein said DNA is transmitted through a complete sexual cycle of said transgenic plant to its progeny, and imparts insect resistance thereto.
Dependencies are inferred from the claim text.