Back to results
US 5,536,646 AGranted
Simplified extraction method for bacterial antigens using dried reagents
AssigneeQuidel Corporation
InventorsTheodore T. Sand, James A. Gordon, Allan D. Pronovost
Life of this patent
Estimating the term…
Abstract
Polysaccharide antigens characteristic of Group A or B Streptococci are extracted for use in immunodiagnostic assays by a simplified method. The method involves the use of two dried reagents, sodium nitrite and Tris base, and a liquid reagent, acetic acid.
Claims10
A method for simultaneously neutralizing and applying an extract of polysaccharide antigens characteristic of Group A or Group B streptococci to a test device which method comprises:
- filtering said extract through a filter material onto the test device wherein said filter material contains neutralizing base or buffer in an amount effective to obtain or maintain the desired pH absorbed and dried thereon;
- wherein said extract has been prepared by extracting a clinical sample suspected of containing said Group A or Group B streptococci with nitrous acid.
Dependencies are inferred from the claim text.