US 5,536,475 AGranted

Apparatus for magnetic cell separation

AssigneeBaxter International Inc.

InventorsAhmad-Maher Moubayed, R. Alan Hardwick

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Abstract

An apparatus for magnetic cell separation using paramagnetic microbeads includes a base upon which is movably carried a rocker member. The rocker member carries both a primary processing container in which a mixture of liquid with cells and paramagnetic microbeads is received; and a primary magnet movable from a first position adjacent to the primary container to magnetically capture the microbeads, and a second position spaced from the primary container to magnetically release the microbeads. The mixture of liquid with cells and microbeads is agitated by tilting motions of the rocker assembly, thereby achieving the binding of target cells to microbeads. The paramagnetic microbeads, with bound target cells, are captured and released by movements of the primary magnet between its first and second positions. A secondary magnet is placed downstream from the primary magnet to capture any paramagnetic microbeads which might escape from the primary magnet. Also provided is an interconnected container apparatus for use to contain and appropriately conduct the liquid, cells, and microbeads.

Claims22

Dependent claims

Apparatus for magnetic cell separation using paramagnetic microbeads and a container set including a primary container which is substantially of elongate cylindrical shade and a secondary container, which containers may be placed in fluid communication with one another, and which containers are magnetically permeable, said apparatus comprising:

a base member, said base member movably supporting an agitation member, and first power drive means for agitating said agitation member relative to said base member;
said agitation member including means for removably securing thereto in a relatively fixed secured position the primary container within which a liquid suspension of the cells and microbeads may be agitated by movement of said agitation member;
said agitation member further movably carrying a primary magnet for movement between a first magnet position adjacent to the primary container in its secured position, in said first magnet position of said primary magnet magnetic flux penetrating the primary container in its secured position to attract and capture the microbeads, and said primary magnet being movable to a second magnet position spaced from the primary container in its secured position so that magnetic flux from said primary magnet does not capture the microbeads;
and second power drive means for selectively moving said primary magnet between said first end second magnet positions independently of agitation of said primary container:
wherein said base member further includes a holder for a secondary container, and a secondary magnet disposed adjacent to said secondary container when the latter is held on said holder for capturing paramagnetic microbeads which escape from said primary container;
wherein said base member pivotally carries said agitation member;
and
wherein said agitation member is configured to secure thereto in a fixed relative position the primary container, said first power drive means also being effective to tiltingly move said agitation member and primary container between a first agitation member position in which the length of the primary container is vertical, and a second agitation member position in which the length of the primary container is horizontal.

5 more elements

Apparatus for performing magnetic cell separation using both paramagnetic microbeads and a container set including an elongate primary container in which cells and paramagnetic microbeads are receivable in liquid mixture, and a secondary container, which first and second containers of said container set are magnetically permeable and may be placed in fluid flow communication with one another, said apparatus comprising:

a base having a tower portion extending upwardly thereon, said tower portion journaling a rocker assembly for pivotal movement about a generally horizontal pivot axis, said rocker assembly including means for securing the elongate primary container thereto in a fixed relative position, said apparatus including a first power drive means both for selectively pivoting said rocker assembly about said pivot axis and for positioning said rocker assembly in selected pivotal positions including a horizontal position and a vertical position for said primary container, a primary magnet having a certain magnetic flux reach carried on said rocker assembly and movable between a first magnet position placing said primary container in its secured position on said rocker assembly within said magnetic reach so that magnetic flux from said primary magnet is effective to magnetically capture a substantial percentage of the paramagnetic microbeads in said primary container, and said primary magnet being movable to a second magnet position spaced from said primary container in its secured position on said rocker member so that said primary container is substantially not within said magnetic flux reach to thereby magnetically release said paramagnetic microbeads to mix into the liquid in the primary container, second power drive means for selectively moving said primary magnet between said first and said second magnet positions, and control means for controlling said first and said second power drive means to selectively agitate said primary container in its secured position on said rocker assembly by opposite tilting movements of said rocker assembly about said pivot axis and to selectively magnetically capture and magnetically release said paramagnetic microbeads in said primary container by respective movements of said primary magnet between its first and second magnet positions.

Dependencies are inferred from the claim text.

Facts

Application: 08/212,479Filed 14 Mar 1994 · continuation in part
Priority: 11 Oct 1988
Granted: 16 Jul 1996
Term ends: Not estimatedThe source records don’t support an estimate.

Classification25 codes

A61M 1/362
Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood…
A61K 35/16
Blood; Artificial blood: blood plasma; Blood serum
A61K 35/28
Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically…:…
A61K 47/51
The non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates: the non-active ingredient being a…

Family

Serial255,214Parent of this continuation-in-part · filed 11 Oct 1988
Serial397,087Parent of this continuation · filed 22 Aug 1989
Serial979,360Parent of this continuation-in-part · filed 20 Nov 1992
Serial187,417Parent of this continuation-in-part · filed 25 Jan 1994
US 5,536,475 AThis patent

From the US filing history. Foreign family members aren’t listed.