Biotransformation of glycopeptide antibiotics
AssigneeEli Lilly and Company
InventorsManuel Debono, Robert M. Logan, Gary G. Marconi, R. Michael Molloy, Milton J. Zmijewski, Jr.
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Abstract
New glycopeptides prepared by biotransformation of a vancomycin-type antibiotic by an Acinomadura citrea culture (NRRL 18382), which are useful intermediates, and methods of preparing the biotransformed intermediates by adding a vancomycin-type glycopeptide to a growing culture of Actinomadura citrea NRRL 18382 and continuing fermentation under submerged aerobic conditions, are provided.
Claims5
A process for bioconverting a vancomycin-type glycopeptide compound of the formula ##STR3## wherein X and Y independently are H or Cl;
- R.sup.2 is H or methyl;
- R.sup.1 is selected from H, vancosaminyl and 4-epi-vancosaminyl;
- R.sup.1a is selected from H and 4-epi-vancosaminyl;
- provided that 1) when R.sup.1a =H, R.sup.1 must be vancosaminyl or H and both X and Y must be Cl;
- and 2) when X=H, R.sup.1 must be 4-epi-vancosaminyl;
- which comprises 1) cultivating Actinomadura citrea NRRL 18382, or a mutant thereof capable of removing D-amino acids from the vancomycin-type glycopeptide compound, in a culture medium containing assimilable sources of carbon, nitrogen, and inorganic salts under submerged aerobic fermentation conditions 2) adding the vancomycin-type glycopeptide compound to the growing culture and 3) continuing the fermentation until a recoverable amount of a bioconverted compound of the formula ##STR4## wherein X, Y, R.sup.1, and R.sup.1a are as hereinabove defined, is produced.
A biologically pure culture of Actinomadura citrea NRRL 18382, or a mutant thereof which removes D-amino acids from vancomycin-type glycopeptide compounds.
Dependencies are inferred from the claim text.