Phenothiazine derivatives, their production and use
AssigneeTakeda Chemical Industries, Ltd.
InventorMarvin A. Motsenbocker
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Abstract
There is disclosed a novel phenothiazine derivative of the general formula (I): ##STR1## wherein R.sub.1, R.sub.2, R.sub.3 and R.sub.4 are the same or different and are a C.sub.1-6 alkyl group and at least one of them has as a substituent a group which can react with amino group, thiol group or carboxyl group, and X.sup.- is a counter ion of the phenazathionium. Their production and the intermediates used in the production are also disclosed. The compound (I) is a derivative of methylene blue and is applicable to photodynamic therapy of cancer or immunoassays utilizing chemiluminescence.
Claims12
A method for determining the concentration of an analyte in a solution, comprising the steps of:
- (a) contacting said solution with a conjugate formed by coupling a binding molecule capable of binding specifically to the analyte to a phenothiazine derivative of general formula (I):
- ##STR12## wherein R.sub.1, R.sub.2, R.sub.3, and R.sub.4 are the same or different and each is a C.sub.1-6 alkyl group, wherein at least one of said alkyl groups is substituted with a substituent so as to react with an amino group, thiol group, or carboxyl group on said binding molecule, and X.sup.- is a counter ion, and
- (b) measuring the amount of said conjugate that binds to said analyte.
A method for determining the concentration of an analyte in a solution, comprising the steps of:
- (a) contacting said solution with (i) a conjugate formed by coupling a sample of said analyte or an analogue of said analyte to a phenothiazine derivative of general formula (I):
- ##STR13## wherein R.sub.1, R.sub.2, R.sub.3, and R.sub.4 are the same or different and each is a C.sub.1-6 alkyl group, wherein at least one of said alkyl groups is substituted with a substituent so as to react with an amino group, thiol group, or carboxyl group on said binding molecule, and X.sup.- is a counter ion, and (ii) a binding molecule capable of binding specifically to both said analyte and said conjugate, and
- (b) measuring the amount of said conjugate that binds to said binding molecule.
Dependencies are inferred from the claim text.