Mutated alpha virus
AssigneeResearch Development Foundation
InventorDennis T. Brown
Life of this patent
Estimating the term…
Abstract
The present invention provides a composition of matter, comprising a non-infectious, mutated virus suitable for use in preparing a vaccine. A plasmid adapted for expression in a recombinant cell comprising DNA encoding non-infectious mutated virus protein and regulatory elements necessary for expression of said DNA in the cell. Also provided are methods of inhibiting the spread of infection of a virus comprising the step of contacting said virus with a compound that inhibits thiol-reductase/protein disulfide isomerase activity in said virus.
Claims12
A non-infectious, mutated alphavirus suitable for use in preparing a vaccine, wherein said virus is mutated by replacing a conserved cysteine amino acid with a different amino acid in the E1 protein and wherein said conserved cysteine is in a domain of the virus protein responsible for thiol-reductase/protein disulfide isomerase activity.
A recombinant DNA plasmid encoding non-infectious mutated alphavirus, wherein said virus is mutated by replacing a conserved cysteine amino acid in the E1 protein with a different amino acid, and wherein said conserved cysteine is in a domain of the virus protein responsible for thiol-reductase/protein disulfide isomerase activity.
Dependencies are inferred from the claim text.