Anti-FR-900506 substance antibodies and highly-sensitive enzyme immunoassay method
AssigneeFujisawa Pharmaceutical Co., Ltd.
InventorsMineo Niwa, Kouichi Tamura, Tsutomu Kaizu, Masakazu Kobayashi
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Abstract
Monoclonal or polyclonal antibodies capable of recognizing at least one antigenic determinant located on the FR-900506 compound, are disclosed. FR-900506 is a compound having pharmacological activities such as immunosuppressive activity and antimicrobial activity, and has the following structure: ##STR1## Also disclosed are enzyme immunoassays for FR-900506 based on the antibodies of the invention and test kits for detection of FR-900506. A process for preparing a monoclonal antibody which selectively binds to FR-900506 is also disclosed.
Claims13
A monoclonal antibody capable of recognizing antigenic determinant(s) located on the FR-900506 substance, which is prepared by:
- i) performing cell fusion between (a) a spleen cell immunized with a FR-900506 substance conjugate with a suitable carrier which is prepared by reacting the suitable carrier with a hydroxyl group on the cyclohexyl moiety of the FR-900506 substance, and (b) a myeloma cell,
- ii) screening the hybridoma producing a monoclonal antibody specific to the FR-900506 substance, and
- iii) obtaining the monoclonal anti-FR-900506 substance antibody, the class of which is IgG,
- in which the FR-900506 substance conjugate with a suitable carrier is the one made by
- a) converting the FR-900506 substance to a half ester of a dicarboxylic acid,
- b) activating its half ester, and
- c) reacting its activated ester with a suitable carrier which can increase the immunogenicity of the FR-900506 substance,
- in which the FR-900506 substance conjugate with a suitable carrier is the one made by reacting the activated ester of FR-900506 substance hemisuccinate of the following formula:
- ##STR4## with a carrier selected from bovine serum albumin, gelatin and hemocyanine.
Dependencies are inferred from the claim text.