Plasmodium vivax blood stage antigens, PvESP-1, antibodies, and diagnostic assays
AssigneeNew York University
InventorJohn W. Barnwell
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Abstract
This invention is directed to novel species-specific P. vivax malarial peptide antigens, PvESP-1 is a protein or fragments of the protein secreted into the plasma of a susceptible mammalian host after infection, and to monoclonal or polyclonal antibodies directed against this antigen. The peptide antigen, monoclonal antibodies, and/or polyclonal antibodies are utilized in assays used to diagnose malaria, as well as to determine whether Plasmodium vivax is the species responsible for the infection.
Claims7
Isolated and purified antibody specifically binding a peptide antigen comprising the amino acid sequence of a species-specific secreted blood-stage protein from P. vivax, or an immunoreactive fragment thereof, said protein being present in detectable amounts in biological fluids of individuals infected with P. vivax malaria, wherein said blood stage protein is PvESP-1.
An assay for the selective identification of P. vivax malarial infection in a susceptible mammal which comprises the steps of:
- (a) contacting a sample of blood or components thereof of said mammal with an antibody that specifically binds to PvESP-1, a P. vivax specific epitope of a species-specific secreted blood-stage protein antigen from P. vivax to form an antibody-antigen complex;
- (b) detecting said complex which indicates whether said mammal is infected by P. vivax.
Dependencies are inferred from the claim text.