Methods and compositions for sequence-specific hybridization of RNA by 2'-5' oligonucleotides
AssigneeDyad Pharmaceutical Corporation
InventorRushdi Alul
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Abstract
(2'-5') linked 3'-Deoxyoligodeoxynucleotides have been synthesized on a solid support via standard cyanoethyl phosphoramidite chemistry. This simple change in the oligonucleotide bond connectivity leads to unique properties. Thus (2'-5') 3'-deoxyoligodeoxynucleotides hybridize selectively to single stranded RNA but not DNA. The (2'-5') linkages confer greater resistance to exo- and endonyucleolytic degradation compared to (3'-5') -linked oligomers. In addition, (2'-5') linked 3'-deoxyoligodeoxynucleotides support RNase-H activity. The nucleic acid selectivity of (2'-5')oligo-3'0-deoxynucleotides may represent an important design feature to improve the efficacy of antisense oligonucleotides.
Claims14
A method of selectively hybridizing an oligonucleotide to RNA comprising hybridizing in a sequence specific manner at least one complementary oligonucleotide to said RNA, said oligonucleotide comprising at least one 2'-5' internucleotide linkage, wherein said oligonucleotide does not hybridize to complementary single stranded DNA in a sequence specific manner.
Dependencies are inferred from the claim text.